The best choice for purification of virus and vaccine——QuikSep Sulfate

2019年10月22日source:管理員
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In the field of biotechnology, affinity chromatography is often used to purify viruses and proteins. Affinity chromatography is a kind of adsorption chromatography, in which the target substances are selectively adsorbed on the ligands of column packing, and then the buffer is changed to achieve separation and recovery. Affinity chromatography is much more accurate than gel filtration or ion exchange chromatography in recovering target components.

QuikSep Sulfate is a specific affinity filler suitable for the isolation and purification of certain viruses, virus-like particles, and certain antigens and proteins (see table 2).


Features:

1. Quick and simple (one-step purification).

2. High load.

3. Easy to enlarge.


1:性能參數

介質

QuikSep Sulfate

基質

高剛性瓊脂糖

粒徑范圍

45-165μm

平均粒徑

90μm

溶菌酶載量

≥3mg/ml?介質

pH穩定性

5-12(長期)?5-12(短期)

操作壓力

≤0.3MPa

流速

16mm× 300mm,0.1MPa300cm/h

貯存溶液

20%乙醇

貯存溫度

4-30

?

2QuikSep?Sulfate適用性

Viruses

Viral/Microbial Agents

Rabies

Influenza ?

Japanese ? Enchephalitis

Feline ? Leukemia

Feline ? Herpes

Feline ? Calicivirus

Respiratory ? Syncytial Virus

Human ? Herpes Simplex

Human ? Measles

Human ? Parainfluenza

Herpes ? Simplex gA and gB Glycoprotein Subunits

Hepatitis ? B Surface Antigen

Filamentous ? Hemagglutinin from B. pertussis

Leucocytosis ? Promoting Factor Hemagglutinin

?

?

QuikSep Sulfate?Purification of rabies virus chromatography

?

1.?The chromatography column

介質:QuikSep Sulfate;?規格:1ml?預裝柱

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2.?Sample pretreatment

高倍濃縮后的樣品用平衡液稀釋?20?倍,上樣前?0.45um?過濾,0.45um?過濾。

?

3.?buffer

A10mM PB,pH 7.2;?B10mM PB,1M NaCl,pH 7.2。

備注:所有溶液需?0.45um?過濾。

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4.?The purification process

流速:1ml/min?或者?30cm/h;

平衡:溶液?A?平衡?10CVCV?指柱體積,下同);

上樣:上樣?30CV;

清洗:溶液?A?平衡?10CV;

洗脫:溶液?B?洗脫?10CV,收集洗脫液。

?

5.?Purification of map

? ? ? ? ? ? ? ? ? ? ? ?QQ截圖20191022082740.jpg


5.?Test results


體積

ml

蛋白濃度

ug/ml

抗原含量

IU/ml

蛋白去除率

%

收率

%

濃縮倍數

純化倍數

原液

30

615

1.51

94

75.4

11

13

流穿液

30

557

0.22

洗脫液

2

531

17.08

?

7.?conclusion

該懸浮培養狂犬病毒純化工藝中,病毒與?QuikSep Sulfate進行吸附結合,洗脫液中蛋白去除率≈94%,回收率≈75.4%?。

8.?Order information

產品

規格(ml)

貨號

QuikSep Sulfate

25

HZ1010-1

QuikSep Sulfate

100

HZ1010-1

QuikSep Sulfate

500

HZ1010-1

QuikSep Sulfate

1000

HZ1010-1


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